Synthetic Biology
based on standard parts
iGEM 2019 Team Information
Team Name: | Fudan-TSI |
Team Code: | 325749 |
Schools: | Fudan University
Shanghai, China
http://www.fudan.edu.cn/en/ |
Kind: | Collegiate | Application Date: 2019-04-01 |
Division: | iGEM | Acceptance Date: 2019-04-03 07:30:46 |
Region: | Asia |
Country: | China |
Section: | Undergraduate
|
Welcome to iGEM 2019 ! Your iGEM 2019 team application was accepted by iGEM Headquarters on 2019-04-03 07:30:46. |
Assigned Track: Foundational Advance
|
R-Evolution: an in vivo sequence-specific toolbox for continuous mutagenesis
|
Mutation library generation is critical for biological and medical research, but current methods cannot mutate a specific sequence continuously without manual intervention. We hereby present a toolbox for in vivo continuous mutation library construction. First, the target DNA is transcribed into RNA. Next, our reverse transcriptase (RT) reverts RNA into cDNA, during which the target is randomly mutated by our RT's enhanced error-prone ability. Finally, the mutated version replaces the original sequence through recombination. These steps will be carried out iteratively, generating a random mutation library of the target with high efficiency as mutations accumulate along with bacterial growth. Our toolbox is orthogonal and provides a wide range of applications among various species. R-Evolution could mutate coding sequences and regulatory sequences, which enables the evolution of individual proteins or multiple targets at a time, promotes high-throughput research, and serves as a foundational advance to synthetic biology.
|
Primary PI [Required]
|
ripple | Daru Lu | |
|
Secondary PI [Recommended]
More...
|
wave | Liang Cai | |
|
Instructors |
None designated
|
Student Leaders
|
WendyZh | Chuyue Zhang | |
|
ZuoleCC | Yuchang Lee | | |
This range of part numbers has been assigned to your team for use during the summer: BBa_K3257000 to BBa_K3257999
|