Synthetic Biology
based on standard parts
iGEM 2022 Team Information
| Team Name: | NJTech_China |
| Team Code: | 411903 |
| Schools: | Nanjing Tech University |
| Kind: | Collegiate | Application Date: 2022-04-04 |
| Division: | iGEM | Acceptance Date: 2022-06-15 11:25:25 |
| Region: | Asia |
| Country: | China |
| Section: | Undergraduate
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Welcome to iGEM 2022 ! Your iGEM 2022 team application was accepted by iGEM Headquarters on 2022-06-15 11:25:25. |
| Assigned Track: Foundational Advance
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Engineering PerR as a switch for the evolution of defenses against oxygen in obligate anaerobes
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| In the distant future, explorers may terraform another planet from anaerobic to aerobic to provide appropriate soil conditions and environments for humanity during interstellar voyages, and the changes of oxygen condition will require engineered soil modification bacteria to perform in both anaerobic and aerobic environments.
In our project, obligate anaerobe Clostridium tyrobutyricum was used as an aerobically modified chassis to explore ways to achieve that goal. Peroxide response regulator, PerR, acting as a key regulator of the molecular oxygen and reactive oxygen species detoxification in Clostridium species, has been proven critical in bacterial oxidation sensitivity.
Our project aims to enhance the oxidative-stress tolerance of C. tyrobutyricum via engineering PerR regulator. The perR gene was deleted and then overexpressed under microaerobic induced promoters like Pvgb. Then, several promoter engineering strategies were employed to achieve the fine regulation of PerR expression.
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| Primary PI [Required]
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| Ling_2022 | Ling Jiang | |
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| Secondary PI [Recommended]
More...
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| NJ | Guannan Liu | |
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| Zhengming | Zhengming Zhu | |
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| Liying_2022 | Liying Zhu | |
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| Instructors |
| None designated
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| Student Leaders |
| None designated |
| This range of part numbers has been assigned to your team for use during the summer: BBa_K4119000 to BBa_K4119999
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