Synthetic Biology

based on standard parts

iGEM 2022 Team Information

Team Name: AFCM-Egypt
Team Code:414082
Schools:
Armed Forces College of Medicine 
Kind:CollegiateApplication Date:  2022-04-04
Division:iGEMAcceptance Date: 2022-06-15 11:25:25
Region:Africa
Country:Egypt
Section:Undergraduate

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TrackMore...
Assigned Track: Diagnostics

Title and AbstractMore...
A Phenylketonuria Diagnostic Platform using Aptamers and E.coli-Based Biosensors along with a Novel Therapeutic Approach.
Phenylketonuria is a metabolic disorder that induces brain damage due to phenylalanine hydroxylase (PAH) deficiency. Herein, we are establishing a diagnostic and therapeutic platform for Phenylketonuria as a prototype for Inborn Errors of Metabolism. The diagnostic approach is achieved via Lateral Flow Assay which provides a cost-effective, accurate, and user-friendly test. We are introducing a novel consumption line in which aptamers-nanoparticles conjugates with pre-specified concentrations consume the normal level of phenylalanine in the sample. The designed aptamers are generated using our directed evolution software tool. Subsequently, the sample passes through the test line that contains a whole cell-based biosensor that releases beta-galactosidase enzyme, providing a semi-quantitative colorimetric signal. This approach uses a CRISPR-regulated therapeutic circuit to control the release of PAH. We introduce a novel Selective Endogenous eNcapsidation for cellular Delivery (SEND) of PAH. Furthermore, safety measures included incorporating anti-CRISPR proteins to control off-targeting actions of Cas proteins.

Team Roster
Primary PI   [Required]
Khaled_ShokryKhaled Shokry
Secondary PI   [Recommended] More...
AymanShawkyAyman Shawky
Instructors
A7madGalalAhmad Mahmoud Galal
AbdelGawadMahmoud Mohammed Mahmoud AbdelGawad
MTarekMohammad Tarek Mansour
MohamedOsama189Mohamed Osama Mohamed Moawad
Dr_BedewyYasser Elbedewy
Ahmed_elshazlyAhmed Elshazly
Tamer_AshryTamer Ashry
Student Leaders
Ahmed_A_RezkAhmed Adel Rezk
Student Members
Ahmed_MattarAhmed Gamal Mohamed Mattar
mahmoud_sayedmahmoud sayed
MhasounaMohamed Sayed Hasouna
Mahmoud_AbdElmonemMahmoud Mohamed Abd-Elmonem
Ahmed_WaelAhmed Wael Mansour
hossHossam Algamal
Khalid21AbdullAzizKhlalid Abdull Aziz Mahdy El-Hela
Omar_EmadOmar Emad Hassan Elsabaye
M-Emad119Mohamed Emad Abd El-Wahab
hossambannisHossam Eldeen Bannis
Mohamed-Ab0elghareMohamed Mohamed Saad Aboelghare
ahmedwa2lAhmed Wael
Mhesham98Mohamed Hesham Abdulhai
Ahmed_ShalanAhmed Shalan
Ahmed_EldahshanAhmed Eldahshan
Advisors
Moetaz_SherifMoetaz Sherif Mohamed Radwan Metawea
MEERO99999Omar Ahmed Abdalla

This range of part numbers has been assigned to your team for use during the summer: BBa_K4140000 to BBa_K4140999