Synthetic Biology
based on standard parts
iGEM 2022 Team Information
Team Name: | AFCM-Egypt |
Team Code: | 414082 |
Schools: | Armed Forces College of Medicine |
Kind: | Collegiate | Application Date: 2022-04-04 |
Division: | iGEM | Acceptance Date: 2022-06-15 11:25:25 |
Region: | Africa |
Country: | Egypt |
Section: | Undergraduate
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Welcome to iGEM 2022 ! Your iGEM 2022 team application was accepted by iGEM Headquarters on 2022-06-15 11:25:25. |
Assigned Track: Diagnostics
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A Phenylketonuria Diagnostic Platform using Aptamers and E.coli-Based Biosensors along with a Novel Therapeutic Approach.
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Phenylketonuria is a metabolic disorder that induces brain damage due to phenylalanine hydroxylase (PAH) deficiency. Herein, we are establishing a diagnostic and therapeutic platform for Phenylketonuria as a prototype for Inborn Errors of Metabolism. The diagnostic approach is achieved via Lateral Flow Assay which provides a cost-effective, accurate, and user-friendly test. We are introducing a novel consumption line in which aptamers-nanoparticles conjugates with pre-specified concentrations consume the normal level of phenylalanine in the sample. The designed aptamers are generated using our directed evolution software tool. Subsequently, the sample passes through the test line that contains a whole cell-based biosensor that releases beta-galactosidase enzyme, providing a semi-quantitative colorimetric signal. This approach uses a CRISPR-regulated therapeutic circuit to control the release of PAH. We introduce a novel Selective Endogenous eNcapsidation for cellular Delivery (SEND) of PAH. Furthermore, safety measures included incorporating anti-CRISPR proteins to control off-targeting actions of Cas proteins.
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This range of part numbers has been assigned to your team for use during the summer: BBa_K4140000 to BBa_K4140999
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