Synthetic Biology
based on standard parts
iGEM 2023 Team Information
Team Name: | LINKS-China |
Team Code: | 480594 |
Schools: | Shenzhen College of International Education; Shenzhen, Guangdong Province, PRC ; https://www.scie.com.cn/
BASIS International School Shenzhen; Shenzhen, Guangdong Province, PRC ; https://bisz.basischina.com/#/home?lang=en
BASIS International School Park Lane Harbour; Huizhou, Guangdong Province, PRC ; https://biph.basischina.com/#/home?lang=en
Tsinglan School; Dongguan, Guangdong Province, PRC |
Kind: | Commercial | Application Date: 2023-04-24 |
Division: | iGEM | Acceptance Date: 2023-05-25 06:12:11 |
Region: | Asia |
Country: | China |
Section: | High_School
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Welcome to iGEM 2023 ! Your iGEM 2023 team application was accepted by iGEM Headquarters on 2023-05-25 06:12:11. |
Assigned Track: High School
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Aroma Anchor
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Ambergris and Sandalwood Oil are both well-known fragrance fixatives since centuries before. However, people hunted and killed sperm whales to obtain ambergris and the growing of sandalwood is highly time and cost consuming. LINKS-China 2023 aims to produce ambrein and santalol, the major components of the fixatives using yeast cell-factory. By engineering S.cerevisiae, we optimized the MVA pathways by over expressing tHMG1 and IDI1. Then, we constructed the pathway of santalene production by expressing ClSS and its mutant. After that, we inserted CYP736A167 and SaCPR2 to convert santalene into santalol. We also constructed a pathway producing ambrein by inserting two different integrations. In the end the yeasts are able to produce 24.23 mg/L of santalene and 40.65 mg/L of santalol, and we have found the most suitable integration for ambrein production. We wrote market analysis to plan the future development of our project. We expect that in the future, LINKS-China will be able to provide society with an economical and high-quality fragrance fixatives.
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This range of part numbers has been assigned to your team for use during the summer: BBa_K4805000 to BBa_K4805999
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